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D7442

Sigma-Aldrich

MTP Taq DNA Polymerase

Taq DNA Polymerase, free of DNA contaminants

NACRES:
NA.55

Quality Level

form

liquid

usage

sufficient for 1500 reactions
sufficient for 250 reactions

feature

Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included: no
hotstart: no

concentration

5 unit/μL

application(s)

PCR: suitable

color

colorless

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5′→3′ DNA polymerase and exonuclease activities, is ∼95 kDa by SDS-PAGE, and has no detectable endonuclease or 3′→5′ exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA.

Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results, especially when targeting conserved sequences, e.g., bacterial 16S rRNA region. Through Sigma′s proprietary DNA removal methods and strict quality control standards, we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to (1) the conserved region of bacterial 16S rRNA, (2) the Taq expression vector, and (3) the human β-actin gene.

While MTP Taq ensures a high-quality, low contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq Buffer with each tube of MTP Taq DNA Polymerase. Each lot of 10× MTP Taq Buffer undergoes the same strict quality control testing as MTP Taq DNA polymerase to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Application

MTP Taq DNA Polymerase has been used:
  • for the amplification of bacterial 16S rRNA genes from purified DNA(33)
  • bacterial genome analysis
  • pathogen detection

MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process that minimizes levels of contaminating DNA. The enzyme has 5′-3′ DNA polymerase and exonuclease activities, is approximately 95 kD by SDS-PAGE, and has no detectable endonuclease or 3′-5′ exonuclease activities. Contaminating DNA present in most other polymerase preparations often precludes or obscures the accurate interpretation of results, especially when targeting conserved sequences (e.g. bacterial 16S rRNA region).

While MTP Taq is a high-quality, low-contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq buffer (Sigma product M 9943) with each tube of MTP Taq DNA Polymerase. Each lot of MTP Taq and 10× MTP Taq buffer undergoes the same strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA Polymerase.

Biochem/physiol Actions

MTP Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands.

Features and Benefits

Low contaminant DNA polymerase
Prevents false positive PCR results from contaminating bacterial DNA

Components

  • MTP Taq DNA Polymerase (D7067)
  • 10x MTP Taq Buffer (M9943)

Unit Definition

One unit incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74 °C.

Other Notes

Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.
Store at –20 °C. For convenience, 10x MTP Taq Buffer can be stored at room temperature.
View more detailed information on MTP Taq DNA Polymerase at www.sigma-aldrich.com/mtptaq.

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
MTP is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificate of Analysis

Certificate of Origin

Li-Hua Chen et al.
Clinical pediatrics, 48(6), 641-647 (2009-05-02)
A method for the detection of bacterial pathogens in sepsis and bacterial meningitis with 16S rRNA gene- based real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) is developed. A total of 190 blood specimens and 5 cerebrospinal fluid specimens from neonates...
Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn?s Disease Susceptibility
Maillard F, et al.
Cells, 8(1), 33-33 (2019)
Yi-Dong Wu et al.
Journal of clinical microbiology, 46(8), 2613-2619 (2008-06-14)
Sepsis is a serious disease with high mortality in newborns. It is very important to have a convenient and accurate method for pathogenic diagnosis of neonatal sepsis. We developed a method of simultaneous detection and Gram classification of clinically relevant...
Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology.
B Cherie Millar et al.
Journal of clinical microbiology, 40(5), 1575-1580 (2002-05-01)
C E Corless et al.
Journal of clinical microbiology, 38(5), 1747-1752 (2000-05-02)
A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with...

Articles

Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase (D7442)

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

Protocols

Low Contaminant Amplification of DNA Using MTP™ Taq DNA Polymerase

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)

Method for PCR amplification of Bacterial DNA with low contamination using MTP Taq DNA Polymerase (D7442)

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