Comparison of expression data requires normalization. The optimum normalization method depends on sample type. The most common method is to normalize to reference genes. It is critical to select appropriate reference genes and validate them, but there are cases when one fails to identify suitable genes for normalization and must use alternatives. There are cases when normalization of a target gene’s expression to another gene’s expression is most unsuitable. In this lecture, we will learn the goals of normalization and how to select the best normalization strategy.
Presented:Wed, December 22, 2021