Dual-Labeled Probes

Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes.

Choose Dual-Labeled Probes for:

• Microarray validation
• Multiply target genes / few samples
• Pathogen detection
• Multiplexing
• Viral load quantification
• Gene expression analysis
• Gene copy determination

Perfect for validating MISSION^® siRNA and shRNA knockdowns.

Benefits of Using Dual-Labeled Probes include:

• Design simplicity for sequence specificity
  
• Extensive availability of reporter/quencher combinations
  
• Increased sensitivity

Add Locked Nucleic Acid to Your Probe for:

• Increased thermal stability and hybridization specificity
• Greater accuracy in SNP detection, allele discrimination, and in vitro quantification or detection
• Easier and more sensitive probe designs for problematic target sequences

How Dual-Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’ end and a quencher molecule located at the 3’ end. The quencher molecule inhibits the natural fluorescence emission of the reporter by fluorescence resonance energy transfer (FRET). The illustration below depicts the mechanism.

The primer is elongated by the polymerase and the probe binds to the specific DNA template. Hydrolysis releases the reporter from the probe/target hybrid, causing an increase in fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.

Product Features include:

• Amounts: 1, 3, 5, and 10 OD
• Purification: HPLC
• Sequence Lengths: 15 - 40 bases
• Quality Control: 100% mass spectrometry
• Format: Supplied dry in amber tubes
• Custom formats available (normalization, special plates, etc.)

Our probes are provided in a format to simplify your experimental planning.

Guaranteed Yields

*Estimate is based on 4 nmoles or 32 µg for 1 OD and 200 nM in 25 µL reaction (5.0 pmol/reaction). Estimate is based on an average sequence length of 25 bases.

Guaranteed OD Yield	Approx. No. of nmoles	Approx. No. of µg	Approx. No. of Reactions*
1	4	32	800
3	12	96	2,400
5	20	160	4,000
10	40	320	8,000

Spectral Properties Table

Dye	Max. EX (nm)	Max. EM (nm)	Compatible Quencher
6-FAM™	495	520	BHQ™-1, TAMRA™
JOE™	529	555	BHQ™-1, TAMRA™
TET™	521	536	BHQ™-1, TAMRA™
Cal Fluor® Gold 5401	522	544	BHQ™-1
HEX™2	535	556	BHQ™-1, TAMRA™
Cal Fluor® Orange 5602	538	559	BHQ™-1
TAMRA™	557	583	BHQ™-2
Cyanine 3	549	566	BHQ™-2
Quasar® 5703	548	566	BHQ™-2
Cal Fluor® Red 5904	569	591	BHQ™-2
ROX™	586	610	BHQ™-2
TxRd (Sulforhodamine 101-X)	597	616	BHQ™-2
Cyanine 5	646	669	BHQ™-3
Quasar® 6705	647	670	BHQ™-3
Cyanine 5.5	675	694	BHQ™-3

¹JOE/TET alternative
²VIC^® alternative
³Cyanine 3 alternative
⁴TAMRA™ alternative
⁵Cyanine 5 alternative

Shipping Schedule

*Delivery times may be longer due to international transit, customs clearance delays, etc. Large projects will be placed on a delivery schedule based upon project needs.

Quantity (OD)	1	3	5	10
Dual-Labeled Probes with Standard Dyes	3-5 days	3-5 days	3-5 days	3-5 days
Dual-Labeled Locked Nucleic Acid Probes with Standard Dyes	7 days	7 days	7 days	7 days
Dual-Labeled Probes with Post-Synthesis Dyes, with/without Locked Nucleic Acid	7-10 days	7-10 days	7-10 days	7-10 days