QuEChERS样品制备方法

QuEChERS是一种分散固相萃取（dSPE）样品制备技术。QuEChERS是“快速、简便、经济、高效、耐用且安全”（Quick, Easy, Cheap, Effective, Rugged and Safe）的简称。此方法最初针对水果和蔬菜农残分析样品制备而开发，现可用于分析其他类型化学物质，包括PAH（多环芳烃）、PCB（多氯联苯）、PBDE（多溴联苯醚）和阻燃剂。这一概念于2002年推出，现已成为分析化学家协会（AOAC 2007.01）和欧洲标准化委员会（EN 15662：2008）官方方法指定内容。

• QuEChERS样品萃取
• QuEChERS纯化

相关技术文章

• Comparison of QuEChERS Products for the Extraction of Planar Pesticides from Spinach
  Supel™ QuE Verde combines a novel carbon with zirconia coated silica (Z-Sep+) to provide an optimum balance between planar pesticide recovery and color removal.
• Analysis of Pesticide Residues in Cannabis Using QuEChERS Extraction and Cleanup Followed by GC/MS/MS Analysis
  Compared to conventional PSA/C18/GCB cleanup, cannabis extracts cleaned with the Supel QuE Verde mixture showed lower GC/MS background, and better recoveries for many pesticides.
• Analysis of Pesticide Residues in Ginger Powder Using QuEChERS Extraction and Clean-up with a Novel Dual Layer SPE Cartridge
  The odor and pungency of ginger is due to the presence of terpenes, gingerols and shogaols. These compounds contribute to the highly complex matrix of ginger, which subsequently presents a challenge in low level analyses of contaminants. A novel 2-layer SPE cartridge efficiently reduces background for pesticide determination.
• Supel™ QuE Z-Sep Sorbents for QuEChERS
  QuEChERs is a solid phase extraction (SPE) technique for sample preparation. Supelco's innovative Z-Sep and Z-Sep+ sorbents demonstrated here extract more fat and pigment than traditional PSA/C18 phases while showing greater analyte recovery and greater reproducibility.
• Improved Recoveries and Lower Background for GC/MS/MS and LC/MS/MS Analysis of Pesticides in Green Tea
  A procedure for cleanup of green tea extracts provides low background and allowed for the analysis of more pesticides at lower levels than QuEChERS. Uses less solvents and no toluene.
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相关实验方案

• Analysis of Active Cannabis Compounds in Edible Food Products: Gummy Bears and Brownies
  Potency testing in marijuana-infused edibles is an important problem that analytical labs are facing due to the complexity of the involved matrices. Concentration of active ingredients in these edibles can range from a few parts per million to 3.5 parts per thousand. This application demonstrates the extraction and HPLC-UV analysis of the active compounds.
• Analysis of Fipronil and Fipronil Sulfone in Eggs, Chicken Meat and Mayonnaise
  A short abstract on a method using QuEChERS and GC/MS/MS for determination of fipronil & fipronil sulfone.
• QuEChERS Method for Pesticide Residue Analysis
  QuEChERS method for pesticide residue analysis requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.
• Extraction and Analysis of Agricultural Pesticides from Oranges Using the “QuEChERS” Method
  Extraction and Analysis of Agricultural Pesticides from Oranges Using the “QuEChERS” Method
• LC/MS/MS Analysis of Pesticide Residues in Pistachios on the Ascentis® Express RP-Amide Column after QuEChERS Extraction
  LC/MS/MS Analysis of Pesticide Residues in Pistachios on the Ascentis® Express RP-Amide Column after QuEChERS Extraction
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QuEChERS样品萃取

溶剂萃取技术旨在最大限度提高基底基质中的分析物得率。此技术使用与水混溶的溶剂（通常为乙腈）和高浓度盐和/或缓冲剂，从样品基质中提取目标分析物。溶剂选择对于减少化合物共萃取非常重要。萃取步骤使用盐实现相分离。这类盐充当缓冲剂调节pH值，并从样品中析出水分。

QuEChERS纯化

样品纯化可减少后续分析过程中的干扰成分，毕竟干扰成分可能损坏分析仪器、扰乱分析物鉴定和定量结果。和传统的SPE管样品纯化方法不同，QuEChERS通过将大量SPE吸附剂与萃取提取物混合，促进纯化。纯化所用的吸附剂可去除与目标分析物共萃取的基质干扰成分。在进行GC-MS/MS或LC-MS/MS分析之前，可利用PSA、C18或GCB（石墨化炭黑）吸附剂去除糖、脂质、固醇、有机酸、蛋白质、类胡萝卜素、叶绿素和其他色素。可能需要专用Z-Sep吸附剂去除含颜料或脂肪的复杂基质。