HPLC Analysis of Peptides and Proteins on Proteomix^® SCX-NP1.7, 5 cm x 4.6 mm I.D. Column

column

Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)

mobile phase

(A) 20 mM sodium phosphate, pH 6.0; (B) A + 1.0 M NaCl

gradient

0 to 75% B in 25 min; 15 min prewash

flow rate

0.75 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

1 mg/mL