HPLC Analysis of Caffeine and Taurine on Ascentis^® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

column

Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

1000 μg/mL in 10:90, water: acetonitrile