跳轉至內容
Merck
  • Global deprivation of brain-derived neurotrophic factor in the CNS reveals an area-specific requirement for dendritic growth.

Global deprivation of brain-derived neurotrophic factor in the CNS reveals an area-specific requirement for dendritic growth.

The Journal of neuroscience : the official journal of the Society for Neuroscience (2010-02-05)
Stefanie Rauskolb, Marta Zagrebelsky, Anita Dreznjak, Rubén Deogracias, Tomoya Matsumoto, Stefan Wiese, Beat Erne, Michael Sendtner, Nicole Schaeren-Wiemers, Martin Korte, Yves-Alain Barde
摘要

Although brain-derived neurotrophic factor (BDNF) is linked with an increasing number of conditions causing brain dysfunction, its role in the postnatal CNS has remained difficult to assess. This is because the bdnf-null mutation causes the death of the animals before BDNF levels have reached adult levels. In addition, the anterograde axonal transport of BDNF complicates the interpretation of area-specific gene deletion. The present study describes the generation of a new conditional mouse mutant essentially lacking BDNF throughout the CNS. It shows that BDNF is not essential for prolonged postnatal survival, but that the behavior of such mutant animals is markedly altered. It also reveals that BDNF is not a major survival factor for most CNS neurons and for myelination of their axons. However, it is required for the postnatal growth of the striatum, and single-cell analyses revealed a marked decreased in dendritic complexity and spine density. In contrast, BDNF is dispensable for the growth of the hippocampus and only minimal changes were observed in the dendrites of CA1 pyramidal neurons in mutant animals. Spine density remained unchanged, whereas the proportion of the mushroom-type spine was moderately decreased. In line with these in vivo observations, we found that BDNF markedly promotes the growth of cultured striatal neurons and of their dendrites, but not of those of hippocampal neurons, suggesting that the differential responsiveness to BDNF is part of a neuron-intrinsic program.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
抗NeuN抗体,克隆A60, clone A60, Chemicon®, from mouse
庫存單位
包裝尺寸
供貨能力
價格
數量
Sigma-Aldrich
Anti-Neuropilin-1 Antibody, Chemicon®, from rabbit
庫存單位
包裝尺寸
供貨能力
價格
數量