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  • SMAD3 Regulates Follicle-stimulating Hormone Synthesis by Pituitary Gonadotrope Cells

SMAD3 Regulates Follicle-stimulating Hormone Synthesis by Pituitary Gonadotrope Cells

The Journal of biological chemistry (2016-12-21)
Yining Li, Gauthier Schang, Ulrich Boehm, Chu-Xia Deng, Jonathan Graff, Daniel J Bernard
摘要

Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.

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Sigma-Aldrich
透明质酸酶 来源于牛睾丸, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
Sigma-Aldrich
2,2,2-三溴乙醇, 97%
Sigma-Aldrich
绒毛膜促性腺激素 人, lyophilized powder, vial of ~2,500 IU
Sigma-Aldrich
多聚甲醛, reagent grade, crystalline
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, 1× crystallized, ≥97% (agarose gel electrophoresis)
Sigma-Aldrich
培养基 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture