SEA-scFv as a bifunctional antibody: construction of a bacterial expression system and its functional analysis.

SEA-scFv as a bifunctional antibody: construction of a bacterial expression system and its functional analysis.

Biochemical and biophysical research communications (1999-03-06)

N Sakurai, T Kudo, M Suzuki, K Tsumoto, S Takemura, H Kodama, S Ebara, A Teramae, Y Katayose, M Shinoda, T Kurokawa, Y Hinoda, K Imai, S Matsuno, I Kumagai

PMID10066451

摘要

A SEA-antibody single chain Fv (SEA-scFv) fusion protein was produced by bacterial expression system in this study. SEA-scFv has both staphylococcal enterotoxin A (SEA) effects and antibody activity directed at the epithelial mucin core protein MUC1, a cancer associated antigen. It was expressed mostly in the cytoplasm as an insoluble form. The gene product was solubilized by guanidine hydrochloride, refolded by conventional dilution method, and purified using metal-chelating chromatography. The resulting SEA-scFv fusion protein preparation was found to react with MUC1 and MHC class II antigens and had the ability to enhance cytotoxicity of lymphokine activated killer cells with a T cell phenotype against a human bile duct carcinoma cell line, TFK-1, expressing MUC1. This genetically engineered SEA-scFv fusion protein promises to be an important reagent for cancer immunotherapy.