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Merck
  • MALDI-TOF to compare polysaccharide profiles from commercial health supplements of different mushroom species.

MALDI-TOF to compare polysaccharide profiles from commercial health supplements of different mushroom species.

Food chemistry (2016-01-19)
Marta López-García, María Sonia Dopico García, José Manuel López Vilariño, María Victoria González Rodríguez
摘要

In this work MALDI-TOF mass spectroscopy was investigated to characterise the β-glucan profiles of several commercial health supplements, without any derivatisation or purification pre-treatment. The effect of two solvents (water and dimethyl sulfoxide) and two MALDI matrices (2,5-dihydroxybenzoic acid and 2',4',6'-trihydroxyacetophenone) was first evaluated on dextran standards. MALDI-TOF was found as a useful and quick technique to obtain structural information of diverse food supplements based on mushroom extracts. The MALDI polysaccharide profiles of 5 supplements from different mushroom species were qualitatively similar showing [Glucan+Na](+) cations with a peak-to-peak mass difference of 16 Da consistent with the repeating unit of the β-(1→3)-glucan. The profiles strongly depended on the sample solvent used, with m/z values around 5000-8000 for water and 2000 for dimethyl sulfoxide; differences between samples were revealed in the molecular weight of the aqueous preparation, with the highest values for Maitake and Cordyceps species.

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通用粘度标准;UKASISO/IEC17025和ISO17034认证, viscosity 5738 mPa.s (25 °C)
通用粘度标准;UKASISO/IEC17025和ISO17034认证, viscosity 990.0 mPa.s (25 °C)