跳轉至內容
Merck
  • [Cu(phen)2](2+) acts as electrochemical indicator and anchor to immobilize probe DNA in electrochemical DNA biosensor.

[Cu(phen)2](2+) acts as electrochemical indicator and anchor to immobilize probe DNA in electrochemical DNA biosensor.

Analytical biochemistry (2015-09-26)
Linlin Yang, Xiaoyu Li, Xi Li, Songling Yan, Yinna Ren, Mengmeng Wang, Peng Liu, Yulin Dong, Chaocan Zhang
摘要

We demonstrate a novel protocol for sensitive in situ label-free electrochemical detection of DNA hybridization based on copper complex ([Cu(phen)2](2+), where phen = 1,10-phenanthroline) and graphene (GR) modified glassy carbon electrode. Here, [Cu(phen)2](2+) acted advantageously as both the electrochemical indicator and the anchor for probe DNA immobilization via intercalative interactions between the partial double helix structure of probe DNA and the vertical aromatic groups of phen. GR provided large density of docking site for probe DNA immobilization and increased the electrical conductivity ability of the electrode. The modification procedure was monitored by electrochemical impedance spectroscopy (EIS). Square-wave voltammetry (SWV) was used to explore the hybridization events. Under the optimal conditions, the designed electrochemical DNA biosensor could effectively distinguish different mismatch degrees of complementary DNA from one-base mismatch to noncomplementary, indicating that the biosensor had high selectivity. It also exhibited a reasonable linear relationship. The oxidation peak currents of [Cu(phen)2](2+) were linear with the logarithm of the concentrations of complementary target DNA ranging from 1 × 10(-12) to 1 × 10(-6) M with a detection limit of 1.99 × 10(-13) M (signal/noise = 3). Moreover, the stability of the electrochemical DNA biosensor was also studied.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
甲醇, ACS reagent, ≥99.8%
Sigma-Aldrich
甲醇, anhydrous, 99.8%
Sigma-Aldrich
甲醇, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
Sigma-Aldrich
甲醇, Laboratory Reagent, ≥99.6%
Sigma-Aldrich
甲醇, Absolute - Acetone free
Sigma-Aldrich
甲醇, BioReagent, ≥99.93%
Sigma-Aldrich
甲醇, ACS spectrophotometric grade, ≥99.9%
Sigma-Aldrich
甲醇, ACS reagent, ≥99.8%
Sigma-Aldrich
甲醇, ACS reagent, ≥99.8%
Sigma-Aldrich
甲醇, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)
Sigma-Aldrich
1,10-菲咯啉 一水合物, ACS reagent, 99%
Sigma-Aldrich
1,10-菲咯啉 一水合物, reagent grade
Sigma-Aldrich
1,10-菲咯啉 一水合物, ACS reagent, puriss. p.a., ≥99.5% (calc. to the dried substance), for redox titration
Sigma-Aldrich
甲醇, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
甲醇, NMR reference standard
Sigma-Aldrich
甲醇-12C, 99.95 atom % 12C