- A two-step method to covalently bind biomolecules to group-IV semiconductors: Si(111)/1,2-epoxy-9-decene/esterase.
A two-step method to covalently bind biomolecules to group-IV semiconductors: Si(111)/1,2-epoxy-9-decene/esterase.
Langmuir : the ACS journal of surfaces and colloids (2009-04-11)
Claire Jeanquartier, Gerburg Schider, Sabine Feichtenhofer, Helmut Schwab, Robert Schennach, Johanna Stettner, Adolf Winkler, Heidrun Gruber-Woelfler, Georg Schitter, Rafael J P Eder, Johannes G Khinast
PMID19360936
摘要
A versatile two-step method has been developed that allows linking of biomolecules covalently to hydrogen-terminated group-IV semiconductors by means of epoxy-alkenes. First, the terminal C==C double bond of the alkene forms a covalent bond with the silicon, germanium, or diamond surface by UV-mediated hydrosilylation. The terminal oxirane moiety then reacts with the biomolecule. As a model system, we investigated the attachment of an esterase B to a Si(111) surface by means of the linker molecule 1,2-epoxy-9-decene. Samples were characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. The immobilized enzyme retained its activity and exhibited good long-term stability.
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