- Poly(ethylene glycol) hydrogel microstructures encapsulating living cells.
Poly(ethylene glycol) hydrogel microstructures encapsulating living cells.
Langmuir : the ACS journal of surfaces and colloids (2002-06-29)
Won-Gun Koh, Alexander Revzin, Michael V Pishko
PMID12088033
摘要
We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 micrometers in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 micrometers allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.
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