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Merck
  • Hydrolysis of synthetic chromogenic substrates by HIV-1 and HIV-2 proteinases.

Hydrolysis of synthetic chromogenic substrates by HIV-1 and HIV-2 proteinases.

Biochemical and biophysical research communications (1990-08-31)
L H Phylip, A D Richards, J Kay, J Kovalinka, P Strop, I Blaha, J Velek, V Kostka, A J Ritchie, A V Broadhurst
摘要

Kinetic constants (Km,Kcat) are derived for the hydrolysis of a number of chromogenic peptide substrates by the aspartic proteinase from HIV-2. The effect of systematic replacement of the P2 residue on substrate hydrolysis by HIV-1 and HIV-2 proteinases is examined.

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Sigma-Aldrich
Lys-Ala-Arg-Val-Nle-p-nitro-Phe-Glu-Ala-Nle amide, ≥97% (HPLC)