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Merck
  • Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy.

Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy.

Nanoscale (2022-08-11)
Rui Han, Waldemar Vollmer, John D Perry, Paul Stoodley, Jinju Chen
摘要

Bacterial mechanical properties (cell wall stiffness and turgor) are important factors for bacterial survival in harsh environments. For an individual bacterial cell, it is challenging to determine the cell wall stiffness and turgor simultaneously. In this study, we adopted a combined finite element modelling and mathematical modelling approach to simultaneously determine bacterial cell wall stiffness and turgor of an individual bacterial cell based on atomic force microscopy (AFM) nanoindentation. The mechanical properties and turgor of Staphylococcus epidermidis, determined by our method are consistent with other independent studies. For a given aqueous environment, bacterial cell wall stiffness increased linearly with an increase in turgor. Higher osmolarity leads to a decrease in both cell wall stiffness and turgor. We also demonstrated that the change of turgor is associated with a change in viscosity of the bacterial cell.

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明胶 来源于猪皮肤, gel strength 80-120 g Bloom, Type A