跳轉至內容
Merck
  • Natterin-like depletion by CRISPR/Cas9 impairs zebrafish (Danio rerio) embryonic development.

Natterin-like depletion by CRISPR/Cas9 impairs zebrafish (Danio rerio) embryonic development.

BMC genomics (2022-02-14)
Ana Carolina Seni-Silva, Adolfo Luis Almeida Maleski, Milena Marcolino Souza, Maria Alice Pimentel Falcao, Geonildo Rodrigo Disner, Monica Lopes-Ferreira, Carla Lima
摘要

The Natterin protein family was first discovered in the venom of the medically significant fish Thalassophryne nattereri, and over the last decade natterin-like genes have been identified in various organisms, notably performing immune-related functions. Previous findings support natterin-like genes as effector defense molecules able to activate multiprotein complexes driving the host innate immune response, notably due to the pore-forming function of the aerolysin superfamily members. Herein, employing a combination of the CRISPR/Cas9 depletion system, phenotype-based screening, and morphometric methods, we evaluated the role of one family member, LOC795232, in the embryonic development of zebrafish since it might be implicated in multiple roles and characterization of the null mutant is central for analysis of gene activity. Multiple sequence alignment revealed that the candidate natterin-like has the highest similarity to zebrafish aep1, a putative and better characterized fish-specific defense molecule from the same family. Compared to other species, zebrafish have many natterin-like copies. Whole-mount in situ hybridization confirmed the knockout and mutant embryos exhibited epiboly delay, growth retardation, yolk sac and heart edema, absent or diminished swim bladder, spinal defects, small eyes and head, heart dysfunction, and behavioral impairment. As previously demonstrated, ribonucleoproteins composed of Cas9 and duplex guide RNAs are effective at inducing mutations in the F0 zebrafish. The considerably high natterin-like copies in zebrafish compared to other species might be due to the teleost-specific whole genome duplication and followed by subfunctionalization or neofunctionalization. In the present work, we described some of the natterin-like features in the zebrafish development and infer that natterin-like proteins potentially contribute to the embryonary development and immune response.

材料
產品編號
品牌
產品描述

Sigma-Aldrich
蛋白酶 来源于灰色链霉菌, Type XIV, ≥3.5 units/mg solid, powder
Roche
抗地高辛-AP,Fab片段, from sheep
Sigma-Aldrich
蛋白酶 K 来源于林伯氏白色念球菌, lyophilized powder, ≥30 units/mg protein