- The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.
The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.
Proceedings of the National Academy of Sciences of the United States of America (2021-01-13)
Jie Liu, Ji Yong Jang, Mehdi Pirooznia, Shihui Liu, Toren Finkel
PMID33431665
摘要
At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should, therefore, be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers.