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Merck

In Vitro and In Vivo Approaches to Assess Rho Kinase Activity.

Methods in molecular biology (Clifton, N.J.) (2017-01-25)
Vincent Sauzeau, Gervaise Loirand
摘要

Increased arterial tone and the resulting rise in peripheral vascular resistance are major determinants of the elevated arterial pressure in hypertension. The RhoA/Rho kinase signaling pathways are now recognized as a major regulator of vascular smooth muscle contraction and arterial tone. Here we describe methods to directly and indirectly assess Rho kinase activity in vitro and in cells and tissues.

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Sigma-Aldrich
Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal, culture supernatant, clone SA19, from rabbit
Sigma-Aldrich
ROKα/ROCK-II Protein, active, rat, 10 µg, Active, rat, N-terminal, His-tagged, fusion protein corresponding to amino acids 2-543 of rat ROKa/ROCK-II. For use in Kinase Assays.