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Merck
  • Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution.

Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution.

Mucosal immunology (2019-08-23)
Mingjuan Wu, Jun Liu, Fanying Li, Shuoya Huang, Jingxin He, Yunxia Xue, Ting Fu, Shanshan Feng, Zhijie Li
摘要

Antibiotics are extremely useful, but they can cause adverse impacts on host bodies. We found that antibiotic treatment altered the composition of the gut microbiota and the gene expression profile in the corneal tissues of postnatal mice and decreased the corneal size and thickness, the angiogenesis of limbal blood vessels, and the neurogenesis of corneal nerve fibers. The reconstitution of the gut microbiota with fecal transplants in antibiotic-treated mice largely reversed these impairments in corneal development. Furthermore, C-C chemokine receptor type 2 negative (CCR2-) macrophages were confirmed to participate in corneal development, and their distribution in the cornea was regulated by the gut microbiota. We propose that the CCR2- macrophage population is a crucial mediator through which gut microbiota affect corneal development in postnatal mice. In addition, probiotics were shown to have the potential effect of restoring corneal development in antibiotic-treated mice. Abx-induced gut dysbiosis has significant, long-term effects on the development of the cornea, and reversal of these suppressive effects takes a long time.

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Sigma-Aldrich
万古霉素 盐酸盐 来源于东方链霉菌, ≥900 μg per mg (as vancomycin base)
Sigma-Aldrich
2-苯基吲哚, technical grade, 95%
Supelco
甲硝唑, analytical standard
Sigma-Aldrich
氨苄西林, meets USP testing specifications
硫酸新霉素, European Pharmacopoeia (EP) Reference Standard