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Key Documents

G005445

HPLC Analysis of Milk Thistle Standards on Ascentis® Express C18

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 20 μg/mL in 80:20, water:methanol

適合性

application for HPLC

應用

vitamins, nutraceuticals, and natural products

分析報告

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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