生物源
mouse
重組細胞
expressed in Hi-5 Insect cells
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
效力
10-20 ng/mL
分子量
80.0 kDa
包裝
pkg of 20 μg
雜質
<0.1 EU/μg endotoxin, tested
顏色
white to off-white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
mouse ... HGF(15234)
一般說明
Hepatocyte growth factor (HGF) is a plasmin related disulfide-linked, hetereodimeric protein containing an α and a β chain. It is synthesized as inactive, single-chain precursor which shares similarity with plasminogen. Its α chain contains an N-terminal hairpin and four kringle domains (NK4).
生化／生理作用
Hepatocyte growth factor (HGF) functions via its receptor c-Met, which gets activated upon tyrosine phosphorylation, and subsequently promotes epithelial cell proliferation, morphogenesis, angiogenesis and motility. It plays a crucial role in organ development, as inactivation of either this gene or its receptor in fetal stages results in organ hypoplasia. Endogenously this protein is involved in the self-repair of various ograns including injured livers, kidneys, lungs etc. HGF is a predominant potent factor involved in lung growth and repair. It functions as a motogen, tubulogen and anti-apoptotic agent on airway epithelial and other cells expressing HGF receptor c-Met. Studies in mice show that HGF determines the severity of Pseudomonas aeruginosa keratitis by enhanced HGF signaling through c-met resulting in elevated pro-inflammatory cytokines and reduced anti-inflammatory cytokines.
外觀
Lyophilized from 10 mM Tris, pH 7.2 + 100mM L-Arginine + 200 mM NaCl.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
HGF signaling impacts severity of Pseudomonas aeruginosa keratitis.
Jiang X et al
Investigative Ophthalmology & Visual Science, 55(4), 2180-2190 (2014)
Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
Raymond WW et al
PLoS ONE, 10(5), e0125797-e0125797 (2015)
L Naldini et al.
The EMBO journal, 10(10), 2867-2878 (1991-10-01)
Scatter Factor (SF) is a fibroblast-secreted protein which promotes motility and matrix invasion of epithelial cells. Hepatocyte Growth Factor (HGF) is a powerful mitogen for hepatocytes and other epithelial tissues. SF and HGF, purified according to their respective biological activities
Kaisa L Hanley et al.
Molecular cancer research : MCR, 20(3), 337-349 (2021-11-24)
The Ras/Erk and NF-κB pathways play critical roles in cell proliferation and are known to drive oncogenesis when overactivated. Herein we report a gatekeeper function of the two pathways by working in synergy to suppress liver tumorigenesis. Hepatocyte-specific deletion of
Jian-Ya Zhou et al.
Cancer letters, 351(2), 265-271 (2014-07-02)
In non-small-cell lung cancer (NSCLC) that harbours an activating epidermal growth factor receptor (EGFR) mutation, over-expression of hepatocyte growth factor (HGF) is an important mechanism involved in the acquired resistance to EGFR-tyrosine kinase inhibitors (TKIs) by restoring activity of the
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