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Merck
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重要文件

SRP3042

Sigma-Aldrich

FGF-acidic human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

同義詞:

ECGF-beta, FGF-1, Fibroblast Growth Factor-acidic, HBGF-1

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

生物源

human

重組細胞

expressed in E. coli

化驗

≥95% (HPLC)
≥95% (SDS-PAGE)

形狀

lyophilized

效力

≥2x10^6 units/mg

分子量

15.8 kDa

技術

cell culture | mammalian: suitable

雜質

<0.1 EU/μg endotoxin, tested

顏色

white to off-white

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... FGF1(2246)

一般說明

FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.

生化/生理作用

FGF-acidic is one of 23 known members of the FGF family. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.

序列

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

外觀

Lyophilized from 5 mM Sodium Phosphate, pH 8.0 + 100 mM NaCl.

重構

Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 8.0 to a concentration of <0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

Fibroblast growth factors.
Ornitz, D.M., et al.
Genome Biology, 2, 30051-300512 (2001)

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