生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
lyophilized powder
分子量
antigen 180 kDa
物種活性
rat, mouse, human
技術
immunohistochemistry: 1:1,000-1:2,000
immunoprecipitation (IP): 3 μL using 200 μg of rat brain
western blot: 1:1,000
UniProt登錄號
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... GRIN2A(2903)
mouse ... Grin2a(14811)
rat ... Grin2a(24409)
一般說明
N-甲基-D-天门冬胺酸(NMDA)受体复合物由NR1和NR2两类亚基组成。仅有一类NR1亚基尚未被鉴定而NR2A、NR2B、NR2C和NR2D这四种不同的NR2受体亚基均已被鉴定。尽管NR2亚基不会单独发挥功能,它们可以与NR1亚基结合以产生一系列不同的受体类型。一系列研究也表明由NR1和NR2亚基形成的受体复合物的功能属性主要是由复合物的NR2成分所决定的。NMDA受体是在突触后的并会在发育和成熟的中枢神经系统(CNS)的可塑性中起重要作用。NMDA受体的激动剂和拮抗剂已被提出可对多种CNS疾病具有治疗作用,包括中风、头部受伤、癫痫、疼痛和阿尔茨海默症。
抗-谷氨酸受体NMDAR2A(NR2A)多克隆兔抗可对NMDA受体的180 kDa NR2A亚基进行标记。该标记可被抗体与免疫原的预吸附所阻断。抗体不会与NR2B或NR2C亚基发生交叉反应。它与大鼠、小鼠和人体组织发生反应。
免疫原
NR2A的C末端部分(1253-1391位氨基酸)。
應用
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析(1篇论文)
蛋白质免疫印迹分析(1篇论文)
抗-谷氨酸受体NMDAR2A(NR2A)多克隆兔抗可通过免疫细胞化学和免疫组织化学(IHC)技术,如免疫印迹和免疫沉淀对NMDA的180 kDa NR2A亚基进行检测和定量。它可作为探针用于确定180 kDa NR2A亚基在N-甲基-D-天门冬胺酸(NMDA)受体复合物的组装和功能中的存在和作用。
外觀
从碳酸氢铵(5 mM)冻干
其他說明
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
Nature communications, 6, 10181-10181 (2015-12-19)
NMDA receptor (NMDAR) composition and synaptic retention represent pivotal features in the physiology and pathology of excitatory synapses. Here, we identify Rabphilin 3A (Rph3A) as a new GluN2A subunit-binding partner. Rph3A is known as a synaptic vesicle-associated protein involved in
The Biochemical journal, 296 ( Pt 3), 877-883 (1993-12-15)
The N-methyl-D-aspartate R1 (NMDAR1) and NMDAR2A subunits were expressed transiently either alone or in combination in human embryonic kidney (HEK) 293 cells. The biochemical and pharmacological properties of the cloned receptors were compared with those of adult rat brain NMDA
International journal of molecular sciences, 23(16) (2022-08-27)
NX210c is a disease-modifying dodecapeptide derived from the subcommissural organ-spondin that is under preclinical and clinical development for the treatment of neurological disorders. Here, using whole-cell patch-clamp recordings, we demonstrate that NX210c increased α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)- and GluN2A-containing N-methyl-D-aspartate
Neurobiology of disease, 32(3), 377-384 (2008-09-09)
Transgenic mice overexpressing Dyrk1A (TgDyrk1A), a Down syndrome (DS) candidate gene, exhibit motor and cognitive alterations similar to those observed in DS individuals. To gain new insights into the molecular consequences of Dyrk1A overexpression underlying TgDyrk1A and possibly DS motor
Scientific reports, 7(1), 66-66 (2017-03-01)
Mutations in the N-methyl-D-aspartate receptor (NMDAR) gene GRIN2A cause epilepsy-aphasia syndrome (EAS), a spectrum of epileptic, cognitive and language disorders. Using bioinformatic and patient data we shortlisted 10 diverse missense mutations for characterisation. We used high-throughput calcium-flux assays and patch
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