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GE17-5087-02

Sigma-Aldrich

HiPrep 26/10 Desalting

Cytiva 17-5087-02, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

同義詞:

Desalting Column, HiPrep Desalting Column

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

材料

polypropylene (PP)

分子量

1000-5000 Da (globular proteins)

包裝

pack of 4

製造商/商標名

Cytiva 17-5087-02

儲存條件

dry media

參數

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

床體積

53 mL

柱長度 × 內徑

10 cm × 26 mm

基質

cross-linked dextran
polymeric

基質活性組

dextran-epichlorohydrin phase

粒徑

17-132 μm (dry)

cleaning

2-13

工作範圍

2-13

分離技術

size exclusion (SEC)

一般說明

HiPrep 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

特點和優勢

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

適合性

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律資訊

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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文章

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條款

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page shows how to prepare samples for purification with affinity chromatography.

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