GE17-5079-02

HiTrap^® rProtein A Fast Flow

Cytiva 17-5079-02, pack of 2 × 1 mL

• 分類程式碼代碼: 23151816
• NACRES: NA.56

屬性

• ligand: recombinant protein A (E. coli)
• 儲存期限: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• 包裝: pack of 2 × 1 mL
• 製造商／商標名: Cytiva 17-5079-02
• 儲存條件: (20% Ehtanol); (20% Ethanol)
• 參數: <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.); 42 psi
• 床尺寸: 7 mm × 25 mm
• 床體積: 1 mL
• 柱內徑: 7 mm
• 基質: 4% cross-linked agarose
• 粒徑: 60-165 μm
• 平均直徑: 90 μm (d50v)
• cleaning: 2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
• 工作範圍: 3-10
• 容量: ~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
• 適合性: suitable for bioprocess medium
• 儲存溫度: 2-8°C

描述

一般說明

HiTrap^® rProtein A FF columns are prepacked with rProtein A Sepharose^™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

應用

The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose^™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

特點和優勢

• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established Protein A-based medium used in many approved MAb processes.
• Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA^® design.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids