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Merck
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Key Documents

G9768

Sigma-Aldrich

鸟苷 5′--三磷酸盐-琼脂糖

aqueous glycerol suspension

同義詞:

5′-GTP agarose

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About This Item

MDL號碼:
MFCD00171655
分類程式碼代碼:
41106500
NACRES:
NA.56

生物源

bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast

品質等級

200

形狀

aqueous glycerol suspension

標籤範圍

1-5 μmol per mL

基質

cross-linked 4% beaded agarose

基質活化

cyanogen bromide

基質結合

ribose hydroxyls

基質墊片

11 atoms (adipic acid dihydrazide)

儲存溫度

−20°C

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相關類別

應用

鸟苷 5′-三磷酸-琼脂糖（5′-GTP 琼脂糖）是甘油水溶液悬浮液中的琼脂糖结合物，用于亲和层析和纯化鸟苷结合蛋白

外觀

悬浮于含有0.25 M NaCl的50％甘油中
鸟苷5′-三磷酸-琼脂糖已被用于：
  • 用于纯化易位刺激因子（translocation stimulating factor）的色谱柱
  • 在GTP-琼脂糖拉下实验（pull down assay）中测量与GTP结合的RHEB（脑Ras同源蛋白，Ras homolog enriched in brain）的水平
  • GTP结合测定中
  • 消耗大鼠肝细胞溶质中的GTP结合蛋白

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

分析證明 (COA)

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P E Glaser et al.
Biochemistry, 34(38), 12193-12203 (1995-09-26)
Recently we demonstrated that the unique stereoelectronic relationships inherent in the structure of plasmenylethanolamine facilitate membrane fusion, and we postulated the existence of a membrane fusion protein which could exploit the propensity of plasmenylethanolamine molecular species to adapt an inverted
Arsenite stress down-regulates phosphorylation and 14-3-3 binding of leucine-rich repeat kinase 2 (LRRK2), promoting self-association and cellular redistribution
Mamais A, et al.
The Journal of Biological Chemistry, 289, 21386-21400 (2014)
Reiaz Ul Rehman et al.
Plant & cell physiology, 49(5), 751-766 (2008-04-04)
The regulatory functions of Rab proteins in membrane trafficking lie in their ability to perform as molecular switches that oscillate between a GTP- and a GDP-bound conformation. The role of tomato LeRab11a in secretion was analyzed in tobacco protoplasts. Green
M Bomsel et al.
The Journal of biological chemistry, 268(34), 25824-25835 (1993-12-05)
In endosomes the polymeric immunoglobulin receptor (pIgR) is sorted into transcytotic vesicles. Transcytosis of the pIgR in polarized Madin-Darby canine kidney (MDCK) cells is regulated both by phosphorylation of Ser664 and by binding of ligand, dimeric IgA (dIgA). Mutation of
Somatic mutations in TSC1 and TSC2 cause focal cortical dysplasia
American Journal of Human Genetics, 100(3), 454-472 (2017)
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