AT-2.1

94101450

• 分類程式碼代碼: 41106514

屬性

• 生物源: rat prostate (ventral)
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat prostatic cancer

細胞系描述

AT-2.1 was developed via in vivo passages of the Dunning R-3327 rat prostatic cancers. A series of other cell lines were produced and they hold in common with Dunning R-3327 and AT-2.1 a structural change in chromosome 4 involving a translocation.

培養基

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/ml. Cells can be easily dislodged from the monolayer mechanically, however 0.25% trypsin or trypsin/EDTA can be used; 5% CO₂; 37°C.

其他說明

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