KG1a

91030101, human bone marrow, Lymphoblast

• 分類程式碼代碼: 41106514

屬性

• product name: KG1a, 91030101
• 生物源: human bone marrow
• 增長模式: Suspension
• 染色體組型: 2n = 46, pseudodiploid
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian bone marrow acute myelogenous leukaemia

細胞系描述

Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

培養基

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO₂; 37°C.

其他說明

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