Clone 81

90031403, feline kidney, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: Clone 81, 90031403
• 生物源: feline kidney
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Cat kidney fibroblast, MSV transformed

細胞系描述

Susceptible to Feline leukaemia virus. There is the possibility of low level secretion of Feline endogenous retrovirus (RD 114). The cells should be handled under laboratory containment level 2.

應用

Virus and cloning studies: FeLV, Feline endogenous retrovirus

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

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