V79-HG04

87020406, hamster lung, Fibroblast-like

• 分類程式碼代碼: 41106514

屬性

• product name: V79-HG04, 87020406
• 生物源: hamster lung
• 增長模式: Adherent
• 染色體組型: Aneuploid
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Hamster Chinese embyro lung fibroblast transformed

細胞系描述

A spontaneously transformed cell line of a primary culture derived from embryonic lung fibroblasts of a Chinese hamster. Thioguanine resistant, HAT sensitive, high cloning efficiency in soft agar and used for HPRT- genotyping selection in HAT.

應用

Cell fusion studies

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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