推薦產品
一般說明
Monoclonal Anti-VSV-G-Peroxidase is a lyophilized preparation of the purified immunoglobulin fraction of monoclonal Anti-VSV-G (mouse IgG1 isotype) isolated from ascites fluid of the P5D4 hybridoma, conjugated to horseradish peroxidase (HRP). The antibody is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide. Vesicular Stomatitis Virus Glycoprotein (VSV-G) exists as a trimer and comprises of four domains, among which domain III is a pleckstrin homology (PH) domain.
特異性
该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中，该抗体将未成熟型VSV-G定位在粗糙内质网（RER）和高尔基复合体潴泡中，将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能，可被P5D4抗体特异性识别，而不与任何内源性蛋白质发生交叉反应。
VSV-G tag sequence (YTDIEMNRLGK) on VSV-G tagged fusion proteins when expressed N- or C-terminal to the fusion protein. Recognizes native and denatured forms of VSV-G tagged proteins.
免疫原
KLH标记的含有水泡性口炎病毒糖蛋白（VSV-G）的15个羧基末端氨基酸（497-511）的合成肽。
應用
Monoclonal Anti-VSV-G−Peroxidase antibody produced in mouse may be used immunoblotting.
生化／生理作用
Vesicular Stomatitis Virus Glycoprotein (VSV-G) amino acid sequence YTDIEMNRLGK, corresponding to amino acids 501-511 has been widely used as an epitope tag in expression vectors, enabling the expression of proteins as VSV-G tagged fusion proteins. It constitutes an attractive model to study maturation and intracellular transport of membrane proteins. VSV-G mediates attachment of VSV to the cell surface and induces pH-dependent fusion between viral and target membranes. Besides, its cytoplasmic domain contains information for several intracellular sorting steps, which include efficient export from the endoplasmic reticulum, basolateral delivery and endocytosis..VSV-G is transported through stacks in the Golgi cisternae.
外觀
Supplied as a lyophilized powder. After reconstitution,the solution contains 1% BSA and 0.05% MIT in 0.01 M sodium phosphate buffered saline, pH 7.4.
重構
Reconstitution with 0.5 mL water results in a solution of 0.01 M sodium phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% MIT.
儲存和穩定性
Stored the lyophilized product at 2-8 °C. After reconstitution, for extended storage, freeze in working aliquots at –20 °C. For continuous use, the solution may be store at 2-8 °C for up to 1 month. Working dilutions should be discarded. Avoid repeated freeze-thaw.
免責聲明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到適合的產品？
試用我們的產品選擇工具.
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
H J Stewart et al.
Gene therapy, 16(6), 805-814 (2009-03-06)
Large-scale production of gene therapeutics comprising equine infectious anaemia virus (EIAV) -based lentiviral vectors (LVs) would benefit from the development of producer cell lines enabling the generation of larger quantities of vector than achievable by transient systems. Such cell lines
Xiangjie Sun et al.
Future virology, 5(1), 85-96 (2010-01-01)
Members of the Rhabdoviridae infect a wide variety of animals and plants, and are the causative agents of many important diseases. Rhabdoviruses enter host cells following internalization into endosomes, with the glycoprotein (G protein) mediating both receptor binding to host
Mary Germino et al.
Yeast (Chichester, England), 23(10), 763-769 (2006-07-25)
Multimeric protein complexes play diverse and vital roles in the cell, but following the composition of these complexes under varying growth conditions can be challenging. Toward that goal, we have designed a vector that permits the double epitope tagging of
Yoann G Santin et al.
Current biology : CB, 29(21), 3707-3713 (2019-10-22)
The type VI secretion system (T6SS) is a multiprotein apparatus that injects protein effectors into target cells, hence playing a critical role in pathogenesis and in microbial communities [1-4]. The T6SS belongs to the broad family of contractile injection systems
Fuping You et al.
Nature immunology, 14(12), 1237-1246 (2013-11-05)
Induction of type I interferon is a central event of innate immunity, essential for host defense. Here we report that the transcription factor ELF4 is induced by type I interferon and upregulates interferon expression in a feed-forward loop. ELF4 deficiency
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務