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A0924

Sigma-Aldrich

丙烯酰胺/N,N'-亚甲基双丙烯酰胺配制试剂

BioReagent, for molecular biology, 49:1 (ratio)

同義詞:

Acrylamide/Bisacrylamide, 49:1 (2.0% crosslinker), Polyacrylamide solution

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About This Item

MDL號碼:
分類程式碼代碼:
41105319
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

產品線

BioReagent

形狀

powder

饋電比

49:1 (ratio)

異物活動

DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected

SMILES 字串

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI 密鑰

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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應用

即复溶干粉混合物精确预混合,得到 40% (w/v) 贮备液,用于蛋白质和核酸电泳。该浓度基于丙烯酰胺和双丙烯酰胺的总重量。
Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

生化/生理作用

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

特點和優勢

无需称量有毒丙烯酰胺和双丙烯酰胺。

重構

向容器中缓慢加入规定量的蒸馏水复溶,并溶解内容物。
To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

標靶器官

Peripheral nervous system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


分析證明 (COA)

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存取文件庫

Bhowmik G
Ana Techniqs in Biotechnology (2011)
PCR Mutation Detection Protocols.
Guilliatt AM
Methods in Molecular Biology (2002)
Preparation and properties of acrylamide gel for use in electrophoresis.
S RAYMOND et al.
Analytical biochemistry, 1, 391-396 (1960-12-10)

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