MABT848

Anti-MYH-2 Antibody, clone 8F72C8

clone 8F72C8, from rat

• 同義詞: Myosin-2, Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain IIa, MyHC-IIa, Myosin heavy chain, skeletal muscle, adult 2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 8F72C8, monoclonal
• 物種活性: rat
• 物種活性（以同源性預測）: mouse, human (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μg
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_001093582
• UniProt登錄號: Q9UKX2
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MYH2(4620)

描述

一般說明

Myosin-2 (UniProt: Q9UKX2; also known as Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain IIa, MyHC-IIa, Myosin heavy chain, skeletal muscle, adult 2) is encoded by the MYH2 (also known as MYHSA2) gene (Gene ID: 4620) in human. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-2 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-784), and an IQ domain (aa 787-816). It also contains 2 actin binding regions (aa 661-683 and 763-777). Mutations in MYH2 gene are known to cause Proximal myopathy and ophthalmoplegia (MYPOP), a muscular disorder characterized by mild-to-moderate muscle weakness and predominance of type 1 fibers and small or absent type 2A fibers.

特異性

Clone 8F72C8 is a rat monoclonal antibody that detects Myosin-2 in mouse and rat muscle. It targets an epitope within 29 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 29 amino acids from the C-terminal half of human Myosin-2

應用

Anti-MYH-2, clone 8F72C8, Cat. No. MABT848, is a rat monoclonal antibody that detects Myosin-2 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected MYH-2 in Immunohistochemistry applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Western Blotting Analysis: A representative lot detected MYH-2 in Western Blotting applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Immunofluorescence Analysis: A representative lot detected MYH-2 in Immunofluorescence applications (Sawano, S., et. al. (2016). PLoS One. 11(11):e0166080).

Immunohistochemistry Analysis: A 1:25 dilution from a representative lot detected MYH-2 in mouse heart and mouse skeletal muscle tissues.

品質

Evaluated by Western Blotting in mouse soleus muscle tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected MYH-2 in mouse soleus muscle tissue lysate.

標靶描述

~220 kDa observed; 223.04 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1