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Key Documents

MABS2320

Sigma-Aldrich

Anti-DNP Antibody, clone 1D10.4

new

同義詞:

DNP, Dinitrophenol

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

1D10.4, monoclonal

分子量

observed mol wt ~66 kDa

純化經由

using protein G

物種活性(以同源性預測)

all

包裝

antibody small pack of 100

技術

ELISA: suitable
western blot: suitable

同型

IgG2bκ

UniProt登錄號

儲存溫度

2-8°C

特異性

Clone 1D10.4 is a mouse monoclonal antibody that detects Dinitrophenol (DNP).

免疫原

KLH-conjugated Dinitrophenol (DNP).

應用

Quality Control Testing

Evaluated by Western Blotting with DNP conjugated to BSA.

Western Blotting Analysis: A 1:500 dilution of this antibody detected DNP-conjugated BSA.

Tested Applications

ELISA Analysis: A representative lot of this antibody detected DNP in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Dinitrophenols (DNPs) are highly toxic synthetic chemical compounds, classified as a mitochondrial uncouplers, as they disrupt the process of oxidative phosphorylation in mitochondria, leading to a significant increase in metabolic rate and heat production. DNPs can prevent adenosine triphosphate (ATP) synthesis, but permit electron transfer to proceed. Beside their commercial use as fungicides, herbicides, and insecticides, DNPs are useful tools to study cellular bioenergetics and the mechanisms of thermogenesis. It can be used as a reactant for catalytic reduction reactions, activation of carboxylic acids by converting them into dinitrophenyl (DNP) esters among others.

外觀

Purified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.

重構

1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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