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Merck
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ALP20

Sigma-Aldrich

Apolipoprotein A-II, human

同義詞:

ApoAII

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.77

生物源

human

品質等級

化驗

>98%

形狀

liquid

製造商/商標名

Chemicon®

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

一般說明

High Density Lipoprotein (HDL) isolated by sequential isopycnic ultracentrifugation are delipidated. Apo A-II is purified by column chromatography in 6M urea (Schonfeld et al. 1977). Purity is determined by quantitative densitometry of SDS polyacrylamide gels (Laemlli 1970). Protein is determined by the Pierce BCA method (which was found to be comparable to the Lowry method) with 1% BSA as the standard. Apo A-II, which is a dimer composed of identical subunits, has a Mr of 17 kD and a pI of 4.9. Apo-II activates hepatic lipase and inhibits LCAT. Apolipoproteins are soluble in aqueous solutions. However, due to its amphipathic structure Apo-II self-associates into oligomers at high protein concentrations. Apo A-II exists as dimers in buffers with 5M guanidine-HCL or 6M urea.

MOLECULAR WEIGHT:

17,400
Product Source: Normal human plasma, fresh, non-frozen. Tested negative for HIV-1, HIV-2, HCV and HBc antibodies and HBsAg.

外觀

Liquid in 10 mM NH4HCO3, pH 7.4. Non-sterile, no preservatives.

儲存和穩定性

Maintain lyophilized material at < -20°C for up to six months. Maintain reconstituted Apo A-II at +4°C for up to 4 weeks. Freezing solutions of apolipoproteins in the absence of denaturants may favor increased self-association or aggregation.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Solubilization of low-density lipoprotein with sodium deoxycholate and recombination of apoprotein B with dimyristoylphosphatidylcholine.
Walsh, M T and Atkinson, D
Biochemistry, 22, 3170-3178 (1983)

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