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Key Documents

AB3381

Sigma-Aldrich

Anti-Myosin Light Chain Antibody, phospho-specific (Ser19)

Chemicon®, from rabbit

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, human

製造商/商標名

Chemicon®

技術

ELISA: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

phosphorylation (pSer19)

基因資訊

human ... MYBPHL(343263)

相關類別

特異性

Myosin, human, smooth and non-muscle regulatory light chain, phosphoSer19. By Western blot the antibody recognizes the ~20 kDa phosphorylated protein. The immunogen sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain. The antibody recognizes monophosphorylated (pSer19) and diphosporylated (pThr18-pSer19) forms of the protein. Reactivity to non-phosphorylated human myosin, smooth and non-muscle regulartory light chain is minimal (less than 1%) by ELISA.

免疫原

Epitope: phospho-specific (Ser19)
Synthetic phosphopeptide from human myosin, smooth and non-muscle regulatory light chain.

應用

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Myosin Light Chain Antibody, phospho-specific (Ser19) is validated for use in ELISA, WB for the detection of Myosin Light Chain.
Western blot: 1:1,000-1:5,000 using ECL.
ELISA: 1:120,000-1:135,000
Immunohistochemistry: 2.5 μg/mL

外觀

Affinity purified immunoglobulin. Liquid in 0.02M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.

儲存和穩定性

Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

In vivo imaging and characterization of actin microridges.
Lam, PY; Mangos, S; Green, JM; Reiser, J; Huttenlocher, A
Testing null
Lei Wei et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(1), 474-493 (2020-01-10)
The RhoA/ROCK-mediated actin cytoskeleton dynamics have been implicated in adipogenesis. The two ROCK isoforms, ROCK1 and ROCK2, are highly homologous. The contribution of ROCK2 to adipogenesis in vivo has not been elucidated. The present study aimed at the in vivo

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