AB10424

Anti-Phospho-Bad (Ser99) Antibody

from rabbit

• 同義詞: BCL-X/BCL-2 binding protein, BCL2-antagonist of cell death protein, BCL2-associated agonist of cell death, BCL2-binding component 6, BCL2-binding protein, Bcl-2-binding component 6, Bcl-2-like 8 protein, Bcl-XL/Bcl-2-associated death promoter
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 技術: western blot: suitable
• NCBI登錄號: NP_116784
• UniProt登錄號: Q92934
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer99)
• 基因資訊: human ... BAD(572)

描述

一般說明

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

特異性

Other species have not been tested.

The antibody reacts with the 18 kDa Phospho-Bad (Ser99) protein.

免疫原

This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.

應用

This Anti-Phospho-Bad (Ser99) Antibody is validated for use in WB for the detection of Phospho-Bad (Ser99).

品質

Western Blot Analysis: 1:250 dilution of this antibody detected Phospho-Bad-S99 on HL60 lysate.

標靶描述

18 kDa

外觀

Format: Purified

分析報告

Control
Western Blot : HL60 lysates.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable