345852

Geranylgeranyltransferase I, GST-Fusion, His•Tag^®, Rat, Recombinant, E. coli

Geranylgeranyltransferase I, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.

• 同義詞: GGTase I
• 分類程式碼代碼: 12352202
• NACRES: NA.54

屬性

• 重組細胞: expressed in E. coli
• 品質等級: 100
• 化驗: ≥90% (SDS-PAGE)
• 形狀: liquid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: wet ice
• 儲存溫度: −70°C

描述

一般說明

Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn²⁺ for optimal activity. The enzyme is purified with Zn²⁺ tightly bound, so addition of Zn²⁺ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.

Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn²⁺ for optimal activity.

生化／生理作用

1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.

警告

Toxicity: Standard Handling (A)

外觀

In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.

重構

Following initial thaw, aliquot and freeze (-70°C).

其他說明

Kalinin, A., et al. 2001. Protein Expr. Purif.22, 84.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable