07-952-S

Anti-trimethyl Histone H3 (Lys79) Antibody, Trial Size

from rabbit, purified by affinity chromatography

• 同義詞: H3K79me2, Histone H3 (dimethyl K79), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: dot blot: suitable; multiplexing: suitable; western blot: suitable
• NCBI登錄號: NP_003484.1
• UniProt登錄號: Q16695
• 運輸包裝: wet ice
• 目標翻譯後修改: trimethylation (Lys79)
• 基因資訊: human ... H3C1(8350)

描述

特異性

Broad species reactivity is expected.

This antibody recognizes Histone H3 methylated at Lys79.

免疫原

Epitope: Lys79

Linear Peptide corresponding to human Histone H3 methylated at Lys79.

應用

Dot Blot Specificity Analysis:
AbSurance Histone H3 Antibody Specificity Arrays (Cat. #: 16-667 and 16-665) containing peptides to all key histone modification sites, were probed with Anti-trimethyl Histone H3 (Lys79) Antibody Antibody ; dilution 1:5000. Proteins were visualized using a goat anti-mouse IgG (H+L) conjugated to HRP and a chemiluminescence detection system.


Multiplex Immunoassay
Specificity of interaction of anti-trimethyl histone H3 Lys79 antibody was assayed using the Luminex multiplex immunoassay platform. Anti-trimethyl-Histone H3 (Lys79) diluted to 0.01 mg/ml concentration was incubated with a cocktail of xMAP microspheres conjugated to the following histone H3 peptides, monomethyl Lys79, unmodified Lys79, trimethyl Lys 27, trimethyl Lys 9, trimethyl Lys79 processed and detected using standard xMAP protocols.

Anti-trimethyl Histone H3 (Lys79) Antibody is a rabbit polyclonal antibody for detection of trimethyl Histone H3 (Lys79) also known as H3K79me2, Histone H3 (di methyl K79) & has been validated in WB, Mplex.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

品質

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.

標靶描述

~ 17 kDa

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa acid extract lysate and recombinant Histone H3

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable