05-623-AF647

Anti-RNA polymerase II Antibody, clone CTD4H8, Alexa Fluor^™ 647 Conjugate

clone CTD4H8, from mouse, ALEXA FLUOR^™ 647

• 同義詞: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RNA polymerase II
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 647
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: CTD4H8, monoclonal
• 物種活性: mouse, human
• 物種活性（以同源性預測）: rat (based on 100% sequence homology), yeast (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable
• 同型: IgG1
• NCBI登錄號: NP_000928.1
• UniProt登錄號: P24928
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... POLR2A(5430) ; mouse ... Polr2A(20020) ; rat ... Polr2A(363633)

描述

一般說明

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

特異性

This antibody recognizes Phospho- and non-phospho-RNA polymerase II.

免疫原

Epitope: carboxyl-terminal domain of RNA polymerase II

KLH-conjugated, synthetic peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.

應用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of RNA polymerase II.

品質

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in HeLa cells.

標靶描述

~210-220 kDa observed

外觀

Protein G Purified

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable