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Key Documents

04-932

Sigma-Aldrich

Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal

culture supernatant, clone C16T, from rabbit

同義詞:

calmodulin 1, calmodulin 1 (phosphorylase kinase, delta), phosphorylase kinase, delta subunit

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

culture supernatant

抗體產品種類

primary antibodies

無性繁殖

C16T, monoclonal

物種活性

vertebrates

技術

western blot: suitable

同型

IgG

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... CALM1(801)

一般說明

Calmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.

特異性

Recombinant proteins containing the calmodulin binding protein epitope tag.

免疫原

Epitope: CBP epitope
KLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.

應用

Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
This Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.

品質

Evaluated by Western Blot on lysates from transformed E. coli.
Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.

標靶描述

The molecular weight is related to the tagged protein.

聯結

Replaces: 05-932

外觀

Cultured supernatant with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
Samples containing the calmodulin binding protein epitope tag.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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