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Sigma-Aldrich

QLAM-AβIδ1LVproS-13CH3 Methyl Labeling Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.12

技術

bio NMR: suitable

品質等級

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

QLAM- Aβlδ1LVproS-13CH3 kit has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

應用

For detection of long-range nOes or for the NMR study of large proteins
QLAM- Aβlδ1LVproS-13CH3 is an optimized kit to produce proteins with 13CH3 isotopomer on regio and stereo specific methyl groups of isoleucine, alanine, leucine and valine. This kit has been tested with protein isotopic labeling in E. coli. It is used in NMR studies to detect structurally meaningful long-range and intermolecular NOEs restraints.

包裝

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials


分析證明 (COA)

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Rime Kerfah et al.
Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

文章

Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.

Methyl Isotope-Labeling of Proteins from NMR-Bio

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