酶联免疫吸附测定(ELISA)
ELISA通常在多孔微孔板中进行，属于分子生物学测定法，常用于多肽、蛋白和抗体等各种分子的检测和定量。这种测定方法可检测pg/mL水平的目标分子，同时在基础研究和疾病研究中发挥着重要的作用。
ELISA检测法：抗原-抗体相互作用
ELISA的基本分子成分通常包括酶偶联抗体、固定的目标分子和检测底物。ELISA获取数据的成功率和质量取决于抗体-抗原相互作用的亲和性和特异性，受多种因素影响，包括pH、温度和离子强度等。
ELISA检测法
直接法ELISA先使用直接结合酶标板或间接结合捕获抗体的固定抗原，再使用抗原特异性酶联一抗和检测底物。更常用的间接检测法先使用未偶联一抗，再使用特异性检测一抗的酶联二抗。间接法只有二抗与酶偶联，因而对目标抗原的免疫反应性更强。直接和间接检测法之外，捕获或“夹心”检测法先使用额外的抗原捕获抗体结合微孔板表面，然后类似上述间接法使用一抗和酶联二抗。
相关技术文章
- Conferma™ Sandwich ELISAs are created with a focus on their critical components. Combined with finished kit quality control analysis, explore Conferma™ ELISA validation that provides a high degree of consistency with each lot to give you confidence in your research.
- The inherent immune ability of an animal can be leveraged to generate antibodies that bind to specific molecules.
- The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
- Review a high-throughput ELISA-based method to identify peptide substrates for class-specific and/or enzyme-specific Protein Tyrosine Kinases (PTKs) that can be utilized for the detection of kinase activity both in vivo and in vitro.
- Troubleshoot and optimize ELISAs using this guide that includes solutions to some of the most common sources of problems for assay development.
- 查看完整内容 (19)
相关实验方案
- This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.
- ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.
- Cell Proliferation ELISA, BrdU (colorimetric) Protocol & Troubleshooting
- Measure and compare the activity of proteases with this non-specific protease activity assay using casein. Use this assay as a standardized procedure to determine the activity of proteases for quality control.
- cAMP Assays protocol from cold spring harbor
- 查看完整内容 (6)
登入以繼續
若要繼續閱讀，請登入或建立帳戶。
還沒有帳戶？