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HomePlant Tissue CultureClassical Plant Media Formulations

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today. Below, find formulation information for these commonly used media.

Component (mg/L)C1416D6162G5768G5893H2395M6774M5524Q3376S6765W0876
Aluminum chloride • 6H2O
Ammonium nitrate 1416.0 400.01650.0400.0
Ammonium phosphate monobasic 115.03 300.0
Ammonium sulfate463.0 134.0134.0
Boric acid1.64.83.03.02.866.26.26.25.01.5
Calcium chloride anhydrous125.33112.5113.24113.24 72.5332.2 151.0
Calcium nitrate 1367.0 656.4386.0 833.77 200.0
Calcium phosphate tribasic
Cobalt chloride • 6H2O 0.0250.025 0.0250.0250.1
Cupric sulfate • 5H2O 0.250.0250.0250.080.250.0250.0250.2
Na2-EDTA37.2545.437.337.25 37.337.2637.320.0
ZnNa2-EDTA
Ferric chloride • 6H2O
Ferric citrate
Ferric sulfate 2.5
Ferric tartrate • 2H2O 5.32
Ferrous sulfate • 7H2O27.8533.827.827.85 27.827.827.815.0
Magnesium sulfate90.37361.49122.09122.09240.76180.7180.7175.79195.4360.0
Manganese chloride • 4H2O 1.81
Manganese sulfate • H2O3.3333.510.010.0 22.316.90.7610.05.04
Molybdenum trioxide 0.016
Molybdic acid (sodium salt) • 2H2O 0.390.250.25 0.250.250.250.1
Nickel chloride • 6H2O
Nickel sulfate • 6H2O 0.005
Potassium chloride 65.0
Potassium iodide0.8 0.750.75 0.830.081.00.75
Potassium nitrate2830.0 2500.02500.0606.6 1900.01800.02500.080.0
Potassium phosphate monobasic400.0265.0 170.0170.0270.0
Potassium sulfate 1559.0 990.0
Sodium nitrate
Sodium phosphate monobasic 130.5130.5 16.5
Sodium sulfate 200.0
Zinc nitrate • 6H2O 17.0
Zinc sulfate • 7H2O1.5 2.02.00.228.68.68.61.02.67
Organics (mg/L)C1416D6162G5768G5893H2395M6774M5524Q3376S6765W0876
Activated charcoal
Adenine hemisulfate
Agar
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma-
Dimethylallylaminopurine
Glycine (free base)
Indole-3-acetic acid
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol 100.0
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid) 1.0
Peptone
Pyridoxine • HCl 1.0
Sucrose
Thiamine • HCl 10.0
C1416D6162G5768G5893H2395M6774M5524Q3376S6765W0876
Grams of powder to prepare 1 L4.05.23.13.21.62.34.33.63.20.934
pH ± 0.5 at room temperature4.14.14.04.04.73.93.93.94.24.7
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