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HomeGC Analysis of Pyrazines in Peanut Butter on SUPELCOWAX® 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

GC Analysis of Pyrazines in Peanut Butter on SUPELCOWAX® 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

Conditions

Sample/Matrix5 g peanut butter in 40 mL vial
Desorption Process270 °C for 5 min
ExtractionHeadspace, 30 min at 65 °C in heating block
SPME FiberDivinylbenzene-Carboxen-polydimethylbenzene StableFlex™ (57328-U)
ColumnSUPELCOWAX® 10, 30 m x 0.25 mm, 0.25 µm (24079)
Oven40 °C (5 min) to 230 °C at 4 °C/min
Carrier GasHelium, 30 cm/sec
SamplePeanut butter
InjectionSplitless/split, closed 0.5 min, 270 °C, with 0.75 mm liner
Liner0.75 mm
Inj. Temp.270 °C
DetectorIon trap mass spectrometer, m/z =30-350 at 0.6 sec/scan. Selected ions used for quantitation.
Table 1

Description

Analysis NoteIn this application, SPME was used for the analysis of volatiles in multifloral honey using a headspace method. Headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX® 10 GC column provides excellent separation of these polar compounds.
CategoriesAnalytical Chromatography, SPME Applications, GC Applications, Foods, Flavors & Fragrances, Volatiles
Featured IndustryAgriculture
Food and Beverages
Legal InformationSUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
Other NotesComplementary Products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
SuitabilityApplication for SPME, Application for GC
Table 2

Analyte

  • 1-Methylpyrrole
  • 2,3-Diethylpyrazine
  • 2,3-Dimethylpyrazine
  • 2,5-Dimethylpyrazine
  • 2,6-Diethyl pyrazine
  • 2,6-Dimethylpyrazine
  • 2-Allyl-5-methylpyrazine
  • 2-Ethenyl-5-methylpyrazine
  • 2-Ethenyl-6-methylpyrazine
  • 2-Ethyl pyrazine
  • 2-Ethyl-3,5-dimethyl pyrazine
  • 2-Ethyl-3-methyl pyrazine
  • 2-Ethyl-5-methyl pyrazine
  • 2-Ethyl-6-methyl pyrazine
  • 2-Methyl-5-isopropylpyrazine
  • 2-Methyl-5-propylpyrazine
  • 2-Methyl-6-cis propenylpyrazine
  • 2-Methylpyrazine
  • 3,5-Diethyl-2-methyl pyrazine
  • 3-Ethyl-2,5-dimethylpyrazine
  • 3-Methylbutanal
  • 4-Methylpentene-2-one
  • 5-Methyl-2-propylpyrazine
  • Carbon disulfide
  • Dimethyl disulfide
  • Heptanal
  • Hexanal
  • Pentanal
  • Trimethyl pyrazine
Materials
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