US EPA Method 632: HPLC Analysis of Pesticides on Ascentis^® C18

column

Ascentis C18, 25 cm x 4.6 mm I.D., 5 μm particles (581325-U)

mobile phase

[A] water; [B] acetonitrile

gradient

10 to 50% B in 13 min; to 60% B in 10 min; to 90% B in 6 min; held for 5 min

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 210 nm

injection

10 μL

sample

as indicated in 16% acetonitrile in water