HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)
Materials
standard
Product No.
Description
Pricing
Trypsin inhibitor from Glycine max (soybean)
solution, sterile-filtered, suitable for, suitable for cell cultureTrypsin inhibitor from chicken egg white
Type II-O, Partially purified ovomucoid, containing ovoinhibitorTrypsin Inhibitor from Glycine max (soybean)
BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)CONDITIONS
column
Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase
[A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate
0.5 mL/min
detector
UV, 280 nm
injection
10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
Description
Analysis Note
The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany