LC/MS Analysis of Drugs of Abuse on Ascentis^® Express RP-Amide

column

Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 10 mM ammonium formate in water; [B] 10mM ammonium formate in acetonitrile; (75:25, A:B)

flow rate

0.2 mL/min

column temp.

35 °C

detector

ESI(+)

injection

2 μL

sample

500 ng/mL in water