HPLC Analysis of Withania Compounds on Ascentis® Express Phenyl-Hexyl
Materials
analytical column
Product No.
Description
Pricing
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 10 cm × 2.1 mmCONDITIONS
column
Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase
[A] water; [B] acetonitrile
gradient
20 to 100% B in 10 min; held at 100% B for 0.5 min
flow rate
0.3 mL/min
pressure
2321 psi (160 bar)
column temp.
35 °C
detector
UV, 227 nm
injection
5 μL
sample
20 μg/mL in water: methanol (80:20)
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express Phenyl-Hexyl for the efficient separation of withania standards.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany