Characterization of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) activity in mouse peritoneal cavity cells.

Characterization of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) activity in mouse peritoneal cavity cells.

Cell biochemistry and function (2017-09-06)

Dhébora Albuquerque Dias, Bruna de Barros Penteado, Lucas Derbocio Dos Santos, Pedro Mendes Dos Santos, Carla Cardozo Pinto Arruda, Maria Rosa Chitolina Schetinger, Daniela Bitencourt Rosa Leal, Jeandre Augusto Dos Santos Jaques

PMID28871607

ABSTRACT

This study aimed to characterize the activity of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5) in peritoneal cavity cells from BALB/c mice. E-NTPDase was activated in the presence of both calcium (1.5mM) and magnesium (1.5mM) ions. However, the activity was higher in the presence of Ca

MATERIALS

Product Number

Brand

Product Description

U4125

Sigma-Aldrich

Uridine 5′-(trihydrogen diphosphate) sodium salt from Saccharomyces cerevisiae, 95-100%

A2153

Sigma-Aldrich

Bovine Serum Albumin, lyophilized powder, ≥96% (agarose gel electrophoresis)

O3125

Sigma-Aldrich

Ouabain octahydrate, ≥95% (HPLC), powder

75351

Sigma-Aldrich

Oligomycin A, ≥99% (HPLC)

O0200000

Ouabain, European Pharmacopoeia (EP) Reference Standard